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Literature summary for 2.4.1.226 extracted from

  • Wang, T.T.; Zhu, C.Y.; Zheng, S.; Meng, C.C.; Wang, T.T.; Meng, D.H.; Li, Y.J.; Zhu, H.M.; Wang, F.S.; Sheng, J.Z.
    Identification and characterization of a chondroitin synthase from Avibacterium paragallinarum (2018), Appl. Microbiol. Biotechnol., 102, 4785-4797 .
    View publication on PubMed
Search for more literature for 2.4.1.226

Cloned(Commentary)

Cloned (Comment) Organism
gene acbD, encoded within the presumed capsule biosynthesis gene cluster, functional recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) as soluble protein Avibacterium paragallinarum

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Avibacterium paragallinarum
Fe3+
-
 Avibacterium paragallinarum
Ni2+
-
 Avibacterium paragallinarum
Zn2+
-
 Avibacterium paragallinarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Avibacterium paragallinarum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates slightly Avibacterium paragallinarum
Mg2+ activates moderately Avibacterium paragallinarum
Mn2+ best activating metal ion Avibacterium paragallinarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NDP-glucose + 3-phospho-D-glycerate Avibacterium paragallinarum
-
 NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
NDP-glucose + 3-phospho-D-glycerate Avibacterium paragallinarum ATCC 29545
-
 NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
NDP-glucose + 3-phospho-D-glycerate Avibacterium paragallinarum NCTC 11296
-
 NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?

Organism

Organism UniProt Comment Textmining
Avibacterium paragallinarum D0QYM5 genotype A
-
Avibacterium paragallinarum ATCC 29545 D0QYM5 genotype A
-
Avibacterium paragallinarum NCTC 11296 D0QYM5 genotype A
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Avibacterium paragallinarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information GlcA with a para-nitrophenyl group can serve as the acceptor for ApCS. The enzyme shows a stringent donor tolerance when the acceptor is as small as this monosaccharide. Ligand/substrate interaction analysis via surface plasmon resonance (SPR) analysis. Substrate specificity, overview Avibacterium paragallinarum ?
-
-
additional information GlcA with a para-nitrophenyl group can serve as the acceptor for ApCS. The enzyme shows a stringent donor tolerance when the acceptor is as small as this monosaccharide. Ligand/substrate interaction analysis via surface plasmon resonance (SPR) analysis. Substrate specificity, overview Avibacterium paragallinarum ATCC 29545 ?
-
-
additional information GlcA with a para-nitrophenyl group can serve as the acceptor for ApCS. The enzyme shows a stringent donor tolerance when the acceptor is as small as this monosaccharide. Ligand/substrate interaction analysis via surface plasmon resonance (SPR) analysis. Substrate specificity, overview Avibacterium paragallinarum NCTC 11296 ?
-
-
NDP-glucose + 3-phospho-D-glycerate
-
 Avibacterium paragallinarum NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
NDP-glucose + 3-phospho-D-glycerate
-
 Avibacterium paragallinarum ATCC 29545 NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
NDP-glucose + 3-phospho-D-glycerate
-
 Avibacterium paragallinarum NCTC 11296 NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
UDP-GalNAc + 4-nitrophenyl D-glucuronide
-
 Avibacterium paragallinarum UDP + 4-nitrophenyl 4-O-(N-acetyl-alpha-D-glucosaminyl)-D-glucuronide
-
 ?
UDP-GalNAc + 4-nitrophenyl D-glucuronide
-
 Avibacterium paragallinarum ATCC 29545 UDP + 4-nitrophenyl 4-O-(N-acetyl-alpha-D-glucosaminyl)-D-glucuronide
-
 ?
UDP-GalNAc + 4-nitrophenyl D-glucuronide
-
 Avibacterium paragallinarum NCTC 11296 UDP + 4-nitrophenyl 4-O-(N-acetyl-alpha-D-glucosaminyl)-D-glucuronide
-
 ?

Subunits

Subunits Comment Organism
? x * 111000, about, sequence calculation, x * 100000-130000, recombinant His-tagged enzyme, SDS-PAGE Avibacterium paragallinarum

Synonyms

Synonyms Comment Organism
AcbD
-
 Avibacterium paragallinarum
ApCS
-
 Avibacterium paragallinarum
chondroitin synthase
-
 Avibacterium paragallinarum
More see also EC 2.4.1.175 Avibacterium paragallinarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 37 assay at Avibacterium paragallinarum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 ApCS is a cold-active enzyme Avibacterium paragallinarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 20 75% activity remaining after 10 h, 60% activity remaining after 70 h Avibacterium paragallinarum
30
-
 60% activity remaining after 10 h, 40% activity remaining after 70 h Avibacterium paragallinarum
40 55 inactivation within 5 h Avibacterium paragallinarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Avibacterium paragallinarum

General Information

General Information Comment Organism
physiological function the chondroitin synthase (ApCS) is a bifunctional enzyme catalyzing the elongation of the chondroitin chain by alternatively transferring the glucuronic acid (GlcA) and N-acetyl-D-galactosamine (GalNAc) residues from their nucleotide forms to the non-reducing ends of the saccharide chains Avibacterium paragallinarum